TITLE:
Study of Sperm Apoptosis and Seminal Oxidant Capacity in Infertile Patients with Genital Tract InfectionsEvaluation of the Efficacy of Levofloxacin in Two Therapeutic Protocols
AUTHORS:
Terradas Claudio, Rodriguez Peña Marcelo, Curi Susana, Grasso Ernesto, Nagelberg Alberto
KEYWORDS:
Seminal Tract Infections; Sperm DNA Fragmentation; Nitric Oxide; Levofloxacine
JOURNAL NAME:
Advances in Sexual Medicine,
Vol.2 No.2,
April
19,
2012
ABSTRACT: Introduction: Antibiotics are the principal treatment of seminal tract infections Our objective is to determine the efficacy of levofloxacin in the eradication of genital tract patogens in infertile patients comparing 28 with 56 days of treatment and to elucidate different seminal parameters availables as markers of antibiotic efficacy in seminal improvement. Materials and Methods: We studied prospectively 50 males patients with seminal tract infections. All patients were treated with levofloxacin 500 mg orally along 28 or 56 days. Two seminal analysis were performed before and after treatment an seminal parameters included TUNEL and nitric oxide levels in seminal plasma were measured. Results: We observed significative differences between both diagrams of treatment. Sperm count and motility increased significatively after treatment. But, sperm morphology do not improve after antibiotic. Necrospermy index but not leucocitospermy had been reduced after levofloxacin. Nitric oxide levels have a direct correlation with sperm count but inverse with motility. The rate of apoptosis determined by TUNEL technique in the study population was 30.46%. There was no correlation between apoptosis parameters and necrozoospermy and there was no correlation between sperm cell apoptosis and NO levels. Conclutions: Bacteriologic cure in seminal infection is higher with a scheme of 56 days than 28 days. Treatment with levofloxacin improves seminal parameters in infertile patients with spermatic tract infection. Nitric oxide but not TUNEL is a good predictive factor of antibiotic efficacy.