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Gong, J., Avigan, D., Chen, D., Wu, Z., Koido, S., Kashiwaba, M. and Kufe, D. (1999) Activation of antitumor cytotoxic T lymphocytes by fusion of human dendritic cells and breast carcinoma cells. Proceedings of the National Academy of Sciences USA, 97, 2715-2718. doi:10.1073/pnas.050587197

has been cited by the following article:

• TITLE: In vitro analysis of T cell responses induced by breast tumor cell lysate pulsed with autologous dendritic cells

  AUTHORS: Nowruz Delirezh, Seyed Mohammad Moazzeni, Fazel Shokri, Mohammad Ali Shokrgozar, Morteza Morteza Atri, Hamid Karbassian

  KEYWORDS: Breast; Lysate; Dendritic Cells; T Cell

  JOURNAL NAME: Advances in Bioscience and Biotechnology, Vol.3 No.2, April 18, 2012

  ABSTRACT: In this In vitro study, T cell responses induced by breast tumor cell lysate pulsed monocyte-derived DCs were analyzed in terms of proliferation, specific cytotoxicity and cytokine-release in order to use in immunotherapeutic settings. Nylon wool enriched T lymphocytes from 5 patients with breast cancer stimulated In vitro with tumor cell lysate pulsed monocyte-derived DCs and their proliferation response were analyzed by [3H] thymidine uptake test. Specific cytotoxic activity of tumor antigen primed T cells after three rounds weekly stimulation was evaluated by flow cytometry, and interferon-γ (IFN-γ) and interleukin-4 (IL-4) cytokines release assay was carried out 24 hours after last stimulation in the supernatant of primed T cells using commercially available ELI-SA kits. T cell proliferation assay revealed that tumor cell lysate pulsed DCs could stimulate autologous T cell proliferation response with stimulation indices 4.9 - 30. T cell mediated cytotoxicity assay demonstrated that tumor antigen primed T cells could significantly kill autologous tumor cells more than normal cells (P γ and IL-4 in response to restimulation by antigen pulsed DCs which were dominated by IFN-γ production in 2 and IL-4 production in 3 out of 5 patients. Our result suggested that breast tumor antigen pulsed DCs could elicit effective specific antitumor T cell responses In vitro, therefore, tumor antigen pulsed DC vaccination may be considered as a novel strategy for immunotherapy of patients with breast cancer refractory to standard modalities.