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Das, B.C., Nandwana, N.K., Das, S., Nandwana, V., Shareef, M.A., Das, Y., et al. (2022) Boron Chemicals in Drug Discovery and Development: Synthesis and Medicinal Perspective. Molecules, 27, Article 2615.
https://doi.org/10.3390/molecules27092615

has been cited by the following article:

• TITLE: A Dual-Standard Reversed-Phase HPLC-UV Method for the Simultaneous Identification, Assay, and Impurities Analysis of Phenylboronic Acid: Using 4-Methoxyphenyl Boronic Acid as a Model Compound

  AUTHORS: Weijiang Ying, Maria Castelo, Jiangwei Li, Frank Wu

  KEYWORDS: Identification, Assay, Impurities, RP-HPLC-UV Analysis of Phenylboronic Acid, Dual-Standard

  JOURNAL NAME: American Journal of Analytical Chemistry, Vol.17 No.5, May 28, 2026

  ABSTRACT: Boronic acids (BAs) are versatile building blocks in organic synthesis and the pharmaceutical industry. A dual-standard reverse phase (RP) HPLC-UV method was developed for the simultaneous identification (as monomer), assay (as monomer), and impurities analysis of phenylboronic acid (PBA), using 4-methoxyphenyl boronic acid (4-MPBA) as a model compound. The method utilizes an Ascentis? Express 90 ? ES-Cyano column with 0.025% TFA in both mobile phases (A: water, B: methanol). It employs a simple gradient with an injection volume of 10 μL and a flow rate of 0.8 mL/min. This QC-friendly, single-sequence method achieves baseline separation of the analyte and its impurities. By utilizing a stable, readily available bromo-analog surrogate for quantitation, it bypasses the need for a single-component primary BA reference standard.