TITLE:
An Integrated Single-Cell Transcriptomic Atlas of Human Tricuspid and Aortic Valve Diseases
AUTHORS:
Jingyi Wang, Mingke Wu, Dingjie Xu, Rong Jin, Jie Hao, Xin Wang, Qing Ge
KEYWORDS:
Single-Cell RNA Sequencing, Tricuspid Regurgitation, Calcific Aortic Valve Disease, Valvular Interstitial Cells, Valvular Endothelial Cells
JOURNAL NAME:
Journal of Biosciences and Medicines,
Vol.14 No.5,
May
26,
2026
ABSTRACT: Tricuspid regurgitation (TR) and calcific aortic valve disease (CAVD) are two common valvular heart diseases with distinct pathological characteristics. However, a systematic single-cell level comparison of their cellular composition and transcriptional profiles remains limited. In this study, we analyzed publicly available single-cell RNA sequencing (scRNA-seq) datasets from human tricuspid valve (TV) and aortic valve (AV) tissues, including samples from TR and CAVD patients as well as corresponding control samples. A total of 128,817 cells passed quality control (QC) from an initial 138,568 cells across all samples. We constructed a processed single-cell dataset covering major valvular cell populations, including valvular interstitial cells (VICs), valvular endothelial cells (VECs), and immune cell populations. Cell type annotation, gene expression profiles, and basic transcriptional differences across disease and control groups were described. In addition, we summarized differences in inferred cell-cell communication patterns between TR and control TV samples and provided a comparative description between TR and CAVD datasets. The processed expression matrices, cell annotations, and metadata have been deposited in the Zenodo repository and are publicly available for reuse. This study provides a single-cell transcriptomic dataset resource for further exploration of human valvular diseases.