TITLE:
Expression Profile Analysis and ceRNA Network Prediction of the lncRNA OIP5-AS1/miR-145-5p/DNAJA3 Axis in Helicobacter pylori Infection
AUTHORS:
Weifeng Hong, Lu Jiang, Hongyu Wei
KEYWORDS:
Helicobacter pylori, lncRNA OIP5-AS1, DNAJA3, Expression Profile Analysis, ceRNA Network
JOURNAL NAME:
Journal of Biosciences and Medicines,
Vol.14 No.4,
April
17,
2026
ABSTRACT: Background: Helicobacter pylori (H. pylori) infection disrupts mitochondrial homeostasis in gastric epithelial cells. However, the lncRNA-mediated stress responses during early infection remain unclear. Objective: To investigate the expression dynamics and putative competing endogenous RNA (ceRNA) network of lncRNA OIP5-AS1, miR-145-5p, and DNAJA3 during H. pylori infection. Methods: We utilized starBase and TargetScan for interaction predictions. A time-gradient in vitro H. pylori infection model using GES-1 cells was established. Expression levels were quantified via RT-qPCR and Western blotting. Results: Bioinformatics revealed conserved miR-145-5p binding sites on OIP5-AS1 and DNAJA3. In vitro, H. pylori infection time-dependently upregulated OIP5-AS1 and DNAJA3 mRNA (P H. pylori infection significantly alters the OIP5-AS1/miR-145-5p/DNAJA3 expression profile. We candidly acknowledge that this study is strictly an expression profile analysis. Lacking direct physical binding and functional phenotypic evidence, the proposed ceRNA regulatory network serves merely as a preliminary hypothesis requiring extensive future validation.