TITLE:
Analytical and Stability Studies on Medical Cosmetics
AUTHORS:
Manal I. Eid, Mary E. K. Wahba
KEYWORDS:
Panthenol; Spectrophotometry; Spectrofluorimetry; Stability Studies
JOURNAL NAME:
American Journal of Analytical Chemistry,
Vol.3 No.3,
March
29,
2012
ABSTRACT: Two simple and sensitive spectrofluorimetric (method Ι) and spectrophotometric (method ΙΙ) methods have been developed for the determination of some chloride containing toothpastes and panthenol-containing cosmetic preparations respectively. Method Ι is based on quantitative fluorescence quenching of (terbium-salicylate-hexamine ternary complex) by fluoride which could be measured at λem/λex of 547 nm/322 nm. The ΔF-concentration plot was rectilinear over the concentration range of 0.5 - 20 μg/ml. Method ΙΙ depends reaction of panthenol with nitrobenzoxadiazole chloride (NBD-Cl) and measuring the absorbance of the resultant product at 480 nm. The absorbance-concentration plot was rectilinear over the concentration range of 2 - 20 μg/ml.