TITLE:
miRNA-30b-5p Targets P4HA2 to Regulate Osteosarcoma Cell Proliferation, Invasion, Migration, and Apoptosis
AUTHORS:
Wusong Liu, Wei Fu, Tianyi Shen, Wenwen Tang, Changgong Lan
KEYWORDS:
Osteosarcoma, P4HA2, miR-30b-5p
JOURNAL NAME:
Journal of Biosciences and Medicines,
Vol.14 No.2,
February
6,
2026
ABSTRACT: Objective: This study aims to elucidate the mechanism by which the microRNA miR-30b-5p inhibits the proliferation, invasion, and migration of osteosarcoma cells while inducing apoptosis through the regulation of P4HA2. Methods: The MG63 osteosarcoma cell line was transfected with miR-NC and miR-30b-5p mimics to establish negative control and miR-30b-5p up-regulated expression models. Subsequently, various assays were conducted, including the CCK8 assay, Transwell assay, flow cytometry, dual luciferase reporter assay, real-time PCR (RT-PCR), and Western blot analysis. Results: Following the transfection of MG63 cells with miR-30b-5p mimics, the expression of miR-30b-5p was significantly up-regulated compared to the control group (t = 77.53, p = 0.0002). After transfecting the cells with miR-NC and miR-30b-5p mimics for 24, 48, and 72 hours, the CCK8 assay results indicated a marked reduction in proliferative ability relative to the control group (24 h: t = 16, P Conclusion: miR-30b-5p plays a role in inhibiting the proliferation, invasion, and migration of osteosarcoma cells while also inducing apoptosis by targeting and down-regulating P4HA2 expression. However, the experiments were constrained, as they were validated in a single cell line and lacked the most direct evidence obtained through “functional recovery assays. experiments”.