TITLE:
Study on Resonance Rayleigh Scattering Spectra of Mercaptopurine-Cu(II)-Nucleic Acid System
AUTHORS:
Jidong Yang, Yang Li
KEYWORDS:
DNA; Mercaptopurine; Cuprum(II); Resonance Rayleigh Scattering
JOURNAL NAME:
American Journal of Analytical Chemistry,
Vol.3 No.3,
March
28,
2012
ABSTRACT: In pH 4.8 Britton-Robinson (B-R) buffer solution, Mercaptopurine (MP) could react with Cu(II) to form stable chelate compound, a new Resonance Rayleigh scattering (RRS) spectrum generated and enhanced for the binary system which the highest peak located at 453 nm as detection wavelength. But the RRS spectra could be quenched after adding her- ring milt DNA (hsDNA), salmon milt DNA (sDNA) and calf thymus DNA (ctDNA) into the binary system, respect- tively. And the weakened degree of spectra was directly proportional to the concentration of DNA. The reaction product of three nucleic acid system have identical spectral features, their range of linearity for the relation of spectral intensity with concentration respectively are 0.05 - 0.9 μg.mL-1 for hsDNA, 0.1 - 0.9 μg.mL-1 for sDNA and 0.3 - 0.9 μg.mL-1 for ctDNA; their detection limit respectively are 5 ng.mL-1for hsDNA, 6 ng.mL-1for sDNA, 6 ng?mL-1 for ctDNA. So a new method for determination of DNA was developed and successfully applied to determine the content of the DNA in artificial synthetized samples. At the same time the spectral features of absorption spectra and RRS spectra of the three reaction system, and the eligible reaction conditions and influencing factors were investigated in this paper.