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Roko, G., Porada, R., Gdula-Argasińska, J., Piekoszewski, W., Chabi-Sika, K., Krakowska-Sieprawska, A., et al. (2024) Comparison of Supercritical CO₂ Extraction and Pressurized Fluid Extraction for Isolation of Alkaloids from Anacardium Occidentale with the Study of Its Anti-Inflammatory Activity. Journal of Pharmaceutical and Biomedical Analysis, 241, Article ID: 115982.
https://doi.org/10.1016/j.jpba.2024.115982

has been cited by the following article:

• TITLE: Bioactivity of Combretum aculeatum Extracts against Methicillin-Resistant Staphylococcus aureus (MRSA) Clinical Isolates: Phytochemical and Antioxidant Insights

  AUTHORS: Esse Adjoavi Agossou, Christine N’tcha, Gautier Roko, Brice Dangnon, Pamphile Aguessi, Durand Dah-Nouvlessounon, Agossou Damien Pacôme Noumavo, Haziz Sina, Adolphe Adjanohoun, Lamine Baba-Moussa

  KEYWORDS: Combretum aculeatum, MRSA, Phytochemicals, Antioxidant Activity, Antibacterial Activity, Clinical Isolates

  JOURNAL NAME: Journal of Biomedical Science and Engineering, Vol.19 No.1, January 23, 2026

  ABSTRACT: The emergence of methicillin-resistant Staphylococcus aureus (MRSA) poses a major challenge to the treatment of skin and soft tissue infections, particularly in resource-limited countries. Medicinal plants represent a valuable source of alternative antimicrobial agents. This study investigated the bioactivity of ethanolic and ethyl acetate extracts of Combretum aculeatum leaves, focusing on their phytochemical composition, antioxidant potential, and antibacterial activity against clinical MRSA isolates. Qualitative phytochemical screening revealed the presence of polyphenols, flavonoids, tannins, alkaloids, saponosides, quinonic derivatives, mucilage, and reducing compounds. Quantitative analyses showed comparable total phenolic and condensed tannin contents between extracts, while flavonoids and hydrolysable tannins were significantly higher in the ethanolic extract. Antioxidant activity assessed by DPPH radical scavenging and phosphomolybdenum reduction assays demonstrated substantial and comparable reducing capacities for both extracts. Antibacterial evaluation using the agar disk diffusion method showed that both extracts inhibited the growth of fifty-four clinical MRSA isolates from wounds, pus, abscesses, and boils. Principal component analysis highlighted relationships between strain susceptibility, extract type, and clinical origin. Overall, C. aculeatum leaf extracts exhibit significant antioxidant and anti-MRSA activities, supporting their traditional use and their potential as sources of bioactive compounds for managing resistant infections.