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Denton, J.R., Dermenjian, R.K. and Mao, B. (2014) Development of an Enantiomeric Separation of D & L Valine as Their Corresponding Isoindole Adducts by RP-HPLC for Utilization of the l-Valine toward Pharmaceutically Relevant Materials. Analytical Methods, 6, 6565-6570.
https://doi.org/10.1039/c4ay01069h

has been cited by the following article:

• TITLE: Development of a Reversed-Phase Chiral HPLC Method for Concurrent Chiral, Achiral, Assay, and Identification Analysis of Boc-L-Valine

  AUTHORS: Hua Zhao, Jiangwei Li, Frank Wu

  KEYWORDS: Boc-L-Valine, Reversed-Phase Chiral HPLC, Polysaccharide Chiral Stationary Phase, Achiral Amino-Acid Impurities, Derivatization GC Benchmark

  JOURNAL NAME: American Journal of Analytical Chemistry, Vol.17 No.1, January 21, 2026

  ABSTRACT: Boc-protected amino acids, such as Boc-L-valine, are widely used as key starting materials in the synthesis of pharmaceutical drug substances, necessitating efficient and reliable analytical control strategies. In this work, a streamlined reversed-phase chiral HPLC method was developed for the comprehensive characterization of Boc-L-valine without the need for derivatization. The method enables simultaneous determination of chiral and achiral impurities, assay, and compound identification within a single analytical run. This integrated approach simplifies the analytical workflow, increases throughput, and significantly reduces overall method validation efforts. Moreover, the method provides a robust and versatile platform readily applicable to routine quality control and method development for other Boc-protected or Fmoc-protected amino acids.