TITLE:
Influence of Fetal Calf Serum and Culture Media on the in Vitro Development of Cỏ Goat Embryos
AUTHORS:
Van Khanh Nguyen, Huong Thi Thu Vu, Au Thi Hoang, Yen Thi Kim Pham, Dat Van Le, Nguyen Thi Le Huong, Nguyen Thi Nhien, Nguyen Thi Van Anh, Nguyen Thi Vinh Ha, Lan Doan Pham
KEYWORDS:
In Vitro Cỏ Goat Embryos, Fetal Calf Serum, Culture Media
JOURNAL NAME:
Open Journal of Animal Sciences,
Vol.16 No.1,
December
12,
2025
ABSTRACT: This study examined the effects of fetal calf serum (FCS) and different culture media on the in vitro development of Cỏ goat embryos. In Experiment 1, no statistically significant differences were detected in the cleavage rates of Cỏ goat oocytes cultured in SOFaa medium supplemented with FCS across the 0-hour, 48-hour, and 120-hour post-fertilization time points, as well as in the control group (P > 0.05). In contrast, the 0-hour group exhibited a significantly higher blastocyst formation rate and greater average cell number per blastocyst compared to the other groups (P Experiment 2, based on the findings from Experiments 1, Cỏ goat embryos were cultured in in vitro culture (IVC) medium supplemented with fetal calf serum (FCS) at 0 hours post-fertilization. No statistically significant difference was observed in the cleavage rates of Cỏ goat oocytes following in vitro fertilization between the SOFaa and CR1aa groups (P > 0.05). However, the SOFaa group demonstrated a significantly higher blastocyst formation rate and greater average cell number per blastocyst compared to the CR1aa group (37.04% vs. 24.98% and 136.08 vs. 125.37, respectively; P in vitro production of Cỏ goat embryos.