TITLE:
Construction of Overexpression Vector for Arabidopsis SOC3 and Its Transient Expression in Nicotiana benthamiana
AUTHORS:
Qiang Lin, Jiayu Zeng, Xiaoju Zhong, Zhuojun Li, Hongbin Zhang, Jianzhong Huang
KEYWORDS:
Arabidopsis, TIR-NBS-LRR, Cell Death, Nicotiana benthamiana
JOURNAL NAME:
Journal of Biosciences and Medicines,
Vol.13 No.11,
October
30,
2025
ABSTRACT: [Objective] To clone the TIR-NBS-LRR class SOC3 gene in Arabidopsis thaliana and analyze its subcellular localization and function in both full-length and truncated fragments. [Method] Full-length SOC3 and its segments cDNA were acquired through RT-PCR with mRNA isolated from leaves of Arabidopsis, and their localization and function in Nicotiana benthamiana were investigated employing Agrobacterium-mediated transient expression technology. [Result] Obtained the full-length cDNA of SOC3, which encodes a 1049 amino acid polypeptide with a calculated molecular mass of 118.57 kDa and an isoelectric point of 7.27. Subcellular localization studies revealed that SOC3 and its TIR domain are located in the nucleus, cytoplasm, and cell membrane. In addition, SOC3-TIR alone triggers cell death in N. benthamiana, which cannot be abolished by co-expression of EXO70B1. [Conclusion] SOC3 and SOC-TIR are both located in the plasma membrane, cytoplasm, and nucleus, and SOC-TIR domain has cell-death-inducing activity in N. benthamiana.