TITLE:
Deciphering the Role of Arabidopsis TIR-NBS 2 Receptor in Cell Death Signaling in Nicotiana benthamiana
AUTHORS:
Jiayu Zeng, Qiang Lin, Peng Jia, Kai Chen, Zhuojun Li, Jianzhong Huang
KEYWORDS:
Cell Death, TIR-NBS 2, EXO70B1, Nicotiana benthamiana
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.16 No.10,
October
20,
2025
ABSTRACT: The truncated Toll/interleukin-1 receptor-NBS (TIR-NBS) proteins act significant role in immunity. Previous articles reported an Arabidopsis thaliana resistance protein TIR-NBS 2 (TN2) can arise violent cell death in tobacco (Nicotiana benthamiana) leaves. Nevertheless, the specific mechanism by which this protein strikes cell death is generally poor. Here, we preliminarily dissected the activation mechanism of TN2 immune receptor in N. benthamiana. The outcomes of the transient analysis attested that the TN2-TIR domain itself is enough to cell-death induction. The yellow fluorescent protein (YFP)-tagged TN2 and TN2-TIR were major localized to the cytoplasm, which was considered essential to the function of the TN2 and TN2-TIR. Additionally, the cell-death-inducing activity of TN2 can be inhibited by the full-length EXO70B1 instead of its truncated fragments. These research findings provided some clues for understanding the activation mechanism of TN2.