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P. Auvichayapat, M. Prapochanung, O. Tunkamnerdthai, B. O. Sripanidkulchai, N. Auvichayapat, B. Thinkhamrop, S. Kunhasura, S. Wongpratoom, S. Sinawat and P. Hongprapas, “Effectiveness of Green Tea on Weight Reduction in Obese Thais: A randomized, Controlled Trial,” Physiology & Behavior, Vol. 93, No. 3, 2008, pp. 486-491. doi:10.1016/j.physbeh.2007.10.009

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• TITLE: Inhibitory Effects of Pu-erh Tea Leaves on Mouse Type IV Allergy

  AUTHORS: Katsuko Yamazaki, Kyoji Yoshino, Chihiro Yagi, Toshio Miyase, Mitsuaki Sano

  KEYWORDS: Pu-erh Tea; Type IV Allergy; Cytokines; Theabrownin; Polyphenol

  JOURNAL NAME: Food and Nutrition Sciences, Vol.3 No.3, March 21, 2012

  ABSTRACT: Pu-erh tea is one of post fermented tea manufactured through oxidative maturing by microorganisms. We investigated the preventive effects of pu-erh tea extract on oxazolone-induced type IV allergy in male (ICR) mice. Oral administration of 50 mg/kg water extract of pu-erh tea leaves resulted in significant preventive effects against mouse type IV allergy. The hydrophilic Theabrownin-like fraction (TBW-ND), with a high molecular weight of approximately 12,000 or higher, was prepared from pu-erh tea leaves by a solvent-extraction method, followed by Diaion HP-20 column chro-matography and dialysis. Oral and percutaneous administrations of TBW-ND at doses of 18.7 mg/kg and 0.037 mg/ear also resulted in significant preventive effects, which included preventing increases in levels of the proinflam-matory cytokine interleukin-12. The anti-allergic ingredients in TBW-ND were predicted to be highly complex compounds containing of polyphenols, polysaccharides and/or proteins. The results suggest pu-erh tea leaves could be a beneficial food for protecting against delayed-type allergy.