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M. Tougou, N. Yamagishi, N. Furutani, K. Kaku, T. Shimizu, Y. Takahata, J. Sakai, S. Kanematsu and S. Hidaka, “The Application of the Mutated Acetolactate Synthase Gene from Rice as the Selectable Marker Gene in the Production of Transgenic Soybeans,” Plant Cell Reports, Vol. 28, No. 5, 2009, pp. 769-776. doi:10.1007/s00299-009-0679-1

has been cited by the following article:

• TITLE: The Mutated Acetolactate Synthase Gene from Rice as a Non-Antibiotic Selection Marker for Transformation of Bamboo Cells

  AUTHORS: Shinjiro Ogita, Nanaka Kikuchi, Taiji Nomura, Yasuo Kato

  KEYWORDS: Bamboo; Mutated Acetolactate Synthase Gene; Particle Bombardment; Suspension Culture

  JOURNAL NAME: American Journal of Plant Sciences, Vol.3 No.3, March 19, 2012

  ABSTRACT: Previously, we developed a particle bombardment-mediated transformation protocol in Phyllostachys nigra bamboo by expressing hygromycin phosphotransferase gene (HPT) and neomycin phosphotransferase II gene (NPT II). Although these marker genes could introduce to several tissue cultured organs (e.g. leaves, buds, and calli) of Phyllostachs bamboo species, some organs showed a high susceptibility and/or a low selectivity to hygromycin and kanamycin. In this report, therefore, we describe advantages and technical details for generating stable transgenic bamboo cells using the particle bombardment method with the mutated-acetolactate synthase gene (mALS) from rice (W548L/S627IOsALS) as a non-antibiotic selection marker. A facile and efficient transformation was achieved with the mALS gene and enhanced fluorescent protein gene (mCherry). Approximately 490 and 1400 mCherry-expressing cells/dish/shot in average were observed in both P. bambusoides and P. nigra under fluorescent stereo-microscope. Stable transgenic bamboo cell lines were generated in a selection medium supplemented with 0.1 μM of bispyribac-sodium (BS) as ALS inhibitor. The integration of mALS gene was identified by in vivo ALS enzyme assay and a PCR-restriction fragment length polymerphism (RFLP) based detection procedures.