TITLE:
Molecular Diversity of Kenyan Lablab Bean (Lablab purpureus (L.) Sweet) Accessions Using Amplified Fragment Length Polymorphism Markers
AUTHORS:
Esther N. Kimani, Francis N. Wachira, Miriam G. Kinyua
KEYWORDS:
Lablab purpureus; Molecular Markers; AFLP; Genetic Diversity
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.3 No.3,
March
19,
2012
ABSTRACT: Lablab purpureus (L.) Sweet is a multipurpose legume that combines use as human food and animal feed in addition to serving as a cover crop for soil conservation. In this work, molecular diversity in Lablab purpureus was assessed using amplified fragment length polymorphism markers on fifty Kenyan lablab accessions obtained from farmers’ fields and the Kenya National gene bank. One hundred and eighty polymorphic bands were revealed using fifteen selective primer pairs. The overall mean expected heterozygosity (He) for the five populations was 0.189. Estimates of components of molecular variance revealed that most of the genetic variation resided within populations (99%) and only 1% variance was among the populations, while Principal Coordinate Analysis showed an overlap between accessions from different geographic origins. The UPGMA cluster analysis generated from the distance matrix of the 50 assayed accessions, revealed low diversity among most of the accessions. The low diversity observed may be due to the narrow genetic base for breeding stocks, and extensive exchange of germplasm among smallholder farmers across the country. Results obtained from this study are discussed in light of the need to enhance the genetic management and improvement of this multipurpose crop species.