TITLE:
Impact of Mangifera indica, Lawsonia inermis, and Carica papaya Leaves Powder on Antioxidant Activity in Broiler Chickens
AUTHORS:
Abdul Sattar Shahani, Imdad Hussain Leghari, Nasir Rajput, Ghulam Shabir Barham, Abdul Kabir
KEYWORDS:
Antioxidant Activity, Broiler Chickens, Herbal Supplementation, Immune Response
JOURNAL NAME:
Open Journal of Veterinary Medicine,
Vol.15 No.7,
July
30,
2025
ABSTRACT: This study evaluates the effects of dietary supplementation with Mangifera indica (mango), Lawsonia inermis (henna), and Carica papaya (papaya) leaf powders on antioxidant activity, lipid metabolism, immune response, and meat quality in broiler chickens. Fresh leaves were shade-dried, ground into powder, and incorporated into feed at specific concentrations for 600-day-old chicks divided into five groups (n = 120/group). Key parameters—including blood biochemistry, antioxidant enzyme activity (MDA, SOD, GPx), lipid profiles, and immune markers—were assessed using standardized protocols. Results indicate that mixed herbal supplementation (Group E) demonstrated the highest DPPH radical scavenging activity (45.23% ± 1.48%, p Carica papaya supplementation (Group D) effectively reduced cholesterol (56.00 ± 0.43 mg/dL) and triglycerides (108.33 ± 0.66 mg/dL). Furthermore, immune responses improved, with increased IgG (4.30 ± 0.14 mg/dL), IgM (2.85 ± 0.13 mg/dL), and Newcastle disease antibody titers (7.00 ± 0.22, p