TITLE:
Cytotoxic and Cell Proliferation Activities of Isocoumarin Derivatives Isolated from Phialocephala scopiformis FC-1873
AUTHORS:
Yuji Higashira, Daigo Wakana, Yuji Doi, Hisashi Takeda, Nobutomo Ikarashi, Satoshi Kitaoka, Fumiaki Sato, Hiyori Itagaki, Tsuyoshi Hosoya, Tomoo Hosoe
KEYWORDS:
(+)-Phaeosphaerin A, (S)-6-Demethylkigelin, Cytotoxicity, Cell Proliferation Effect, Phialocephala scopiformis
JOURNAL NAME:
Advances in Microbiology,
Vol.15 No.5,
May
30,
2025
ABSTRACT: Phialocephala scopiformis (Ascomycota, Helotiales, Mollisiaceae) are fungi known as both saprophytes and endophytes, forming small apothecia (1 - 3 mm in diameter) on decaying wood. In this study, three novel isocoumarin derivatives (+)-phaeosphaerin A (1), (+)-phaeosphaerin B (2), and (S)-6-demethylkigelin (3) were isolated from Phialocephala scopiformis FC-1873 collected in Japan, along with two known isocoumarins, lignicol (4) and 6,8-dihydroxy-3,5-dimethyl-1H-2-benzopyran-1-one (5). The cytotoxicity of 1 and 4 were evaluated against HL-60, PANK-1, HepG2, HT-29, and T98G cell lines, as well as their effects on cell proliferation in the skin keratinocyte cell line HaCaT. These findings highlight the potential of mollisioid fungi as a source of novel bioactive compounds.