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K. M. De Antonis, P. R. Brown and S. A. Cohen, “High-Performance Liquid Chromatographic Analysis of Synthetic Peptides Using Deriva-tization with 6-Amino- quinolyl-N-Hydroxysuccinimidyl Car-bamate,” Analytical Biochemistry, Vol. 223, No. 2, 1994, pp. 191-197. doi:10.1006/abio.1994.1572

has been cited by the following article:

• TITLE: Validation of a HPLC Method for Determination of Glutamine in Food Additives Using Post-Column Derivatization

  AUTHORS: Marlus Chorilli, Hérida Regina Nunes Salgado, Fabiana De Santana Santos, Lucélia Magalhães Da Silva

  KEYWORDS: Glutamine; Food Additive Product; Liquid Chromatography

  JOURNAL NAME: American Journal of Analytical Chemistry, Vol.3 No.2, February 22, 2012

  ABSTRACT: An ion-exchange chromatography (IEC) followed by post-column derivatization method was validated for the determination of glutamine in food additive products. The chromatographic method was carried out on a sodium cation-exchange column and using detection at 570 nm. The mobile phase consisted of sodium eluant, pH 3.15, with 5% sulfolane, sodium eluant, pH 7.40 and sodium column regenerant. The method was linear in the range of 4 - 50 μg/mL (r2 = 0.9999). The accuracy was 99.78%. Moreover, method validation demonstrated acceptable results for precision and robustness. The method was found to be able to use for routine analysis of the glutamine.