TITLE:
Recombination and Mutation of a New Chinese Strain of HP-PRRSV SCMY2023
AUTHORS:
Xianchang Zhu, Wenhui Liu, Yang Xia, Zhaoxia Yang, Qian Lei, Yi Zhang, Bo Zhang, Jida Li
KEYWORDS:
PRRSV, Isolation and Identification, Recombinant Virus, Prevention and Control Strategy
JOURNAL NAME:
Journal of Biosciences and Medicines,
Vol.13 No.1,
January
27,
2025
ABSTRACT: In this study, RT-PCR was performed on lung tissue samples from sick pigs in a suspected outbreak of porcine reproductive and respiratory syndrome (PRRS) at a pig farm in Mianyang City, Sichuan Province, China. Positive samples were inoculated into Marc-145 cells to observe lesions. The Marc-145 cells with cytopathic lesions were identified by indirect immunofluorescence. The whole genome sequences of the isolated and purified strains were amplified by RT-PCR and analyzed for homology and genetic evolution. A strain of porcine reproductive and respiratory syndrome virus (PRRSV), named SCMY2023 (GenBank No. PQ179742), was successfully isolated. SCMY2023 has a genome length of 15,321 base pairs (without a poly A tail). Nucleotide and amino acid homology analyses suggest that this strain belongs to Lineage 8, a variant of the highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) prevalent in China. Recombination and genetic evolution analyses indicate that this isolate is a PRRSV variant that recombined with HuN-ZZ (Lineage 8, 98.79% homology) on the backbone of the SCSN2020 strain (Lineage 8, 99.35% homology) in the recombination region from 4407 to 13,107 nucleotides (ORF1a to ORF3). In-depth study of the genetic recombination of this isolate can provide a reference for the prevention and control of PRRS.