TITLE:
Up-Regulation of the Gap Junction Intercellular Communication by Tea Polyphenol in the Human Metastatie Lung Carcinoma Cell Line
AUTHORS:
Xiangyong Li, Qinghua Wang, Jun Yang, Yanjuan Pan, Qingyong Chen, Xiqing Yan, Daxin Wang, Xijian Zhou, Yuquan Wu
KEYWORDS:
Tea Polyphenol; Lung Neoplasms; Highly Metastatic Human Lung Carcinoma Cell Line; Gap Junction Intercellular Communication
JOURNAL NAME:
Journal of Cancer Therapy,
Vol.3 No.1,
February
17,
2012
ABSTRACT: Our previous study has proven that tea polyphenol has a role in lung neoplasms. The present communication was to investage the anti-proliferation effect of tea polyphenol on the PG cells, which was a high metastatic human lung carcinoma cell line, by 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-diphenytetrazoliumromide (MTT) cell viability assay, and to study the change of intracellular calcium concentration, connexin43 (Cx43) expression, gap junctional intercellular communication (GJIC) and cell cycle distribution after the tea polyphenol treatment by laser scanning confocal microscopy and flow cytometry. The results showed that 1) tea polyphenol could kill the PG cells in a dose-depent manner via inhibiting the PG cell proliferation and blocking the PG cell cycle progression staying in G0/G1 phase and not transfering in S and G2/M phases to reduce the PG cell proliferation index; 2) the increases of intracellular calcium concentration, GJIC and Cx43 expression were related with the tea polyphenol doses. The data suggested that tea polyphenol could inhibit the growth of PG cells, which mechanism was associated with the up-regulation of GJIC.