TITLE:
Chiral Method Development Using Polysaccharide-Based Chiral Stationary Phase (CSP) and Investigation of Chiral Column Degradation and Regeneration
AUTHORS:
Hua Zhao, Mary Ababat, Kyle Leeman, John Tucker
KEYWORDS:
Chiral Reversed-Phase HPLC Separation, Backflush, Chiral Column Regeneration, INGREZZA® (Valbenazine)
JOURNAL NAME:
American Journal of Analytical Chemistry,
Vol.15 No.12,
December
27,
2024
ABSTRACT: Two polysaccharide-based chiral stationary phase columns were evaluated to improve the previous partial chiral peak separation to a baseline-resolved separation of the INGREZZA® drug substance and its diastereomers. Moreover, the tailing factor (Tf) variation was studied to investigate chiral column degradation and regeneration and to optimize chiral column performance and efficiency.