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S. F. Altschul, T. L. Madden, A. A. Schaffer, Z. Zhang, W. Miller and D. J. Lipman, “Gapped BLAST and PSI-BLAST: A New Generation of Protein Data Base,” Nucleic Acid Research, Vol. 25, No. 17, 1997, pp. 389-3402. doi:10.1093/nar/25.17.3389

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• TITLE: Molecular Characterization of Pseudomonas spp. Isolated from Root Nodules of Various Leguminous Plants of Shekhawati Region, Rajasthan, India

  AUTHORS: Sakshi Issar, Saroj Sharma, Devendra Kumar Choudhary, Hemant Kumar Gautam, R. K. Gaur

  KEYWORDS: PGPRs; 16S Sequencing; Pseudomonas; Root-Nodule Bacteria; 16S rDNA

  JOURNAL NAME: American Journal of Plant Sciences, Vol.3 No.1, December 31, 2011

  ABSTRACT: Plant growth promontory Pseudomonas strains were isolated from root nodules of five plant species, viz., Trifolium pretense, Cicer arietinum, Amaranthus polygamus, Vigna mungo, and Trigonella foenum; that plants were denizen of Shekhawati region of Rajasthan. A total of 8 bacterial isolates were evaluated for growth promotion using PGP properties. Partial 16S rDNA sequencing data showed that these 8 bacterial isolates belonged to genus Pseudomonas. MEGA 4.0.2, software was used to construct a neighbor joining tree by employing boot strap method. Result exhibited significant diversity among recovered Pseudomonas strains.