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Kim, Y., Hendrickson, R., Mosier, N.S. and Ladisch, M.R. (2009) Liquid Hot Water Pretreatment of Cellulosic Biomass. In: Mielenz, J.R., Ed., Methods in Molecular Biology, Humana Press, 93-102.

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• TITLE: Preparative Fractionation of Feruloyl Oligosaccharides Produced by Combinatorial Enzyme Digestion of Arabinoxylan

  AUTHORS: Dominic W. S. Wong, Sarah Batt, William John Orts

  KEYWORDS: Format, Wheat Insoluble Arabinoxylan, Feruloyl Oligosaccharide, Combinatorial Enzyme Digestion

  JOURNAL NAME: Advances in Enzyme Research, Vol.12 No.3, September 30, 2024

  ABSTRACT: Pretreated wheat insoluble arabinoxylan was converted to oligosaccharides of structural variants using combinatorial enzyme approach. The digestive products were separated by preparative scale chromatographic Amberlite XAD-2 column. Fractions containing feruloyl oligosaccharides (FOS) were isolated, pooled, freeze-dried, and demonstrated to possess antimicrobial activity. The FOS suppressed cell growth of the test organism ATCC 8739 E. coli with a MIC value of 0.028% (w/v, 35˚C, 24 hr). The antimicrobial action was observed exceeding 72 hr of culture incubation. The FOS product could be a useful source of prebiotics or preservatives. The present results further confirm the science and application of the concept of combinatorial enzyme technique.