TITLE:
Preparative Fractionation of Feruloyl Oligosaccharides Produced by Combinatorial Enzyme Digestion of Arabinoxylan
AUTHORS:
Dominic W. S. Wong, Sarah Batt, William John Orts
KEYWORDS:
Format, Wheat Insoluble Arabinoxylan, Feruloyl Oligosaccharide, Combinatorial Enzyme Digestion
JOURNAL NAME:
Advances in Enzyme Research,
Vol.12 No.3,
September
30,
2024
ABSTRACT: Pretreated wheat insoluble arabinoxylan was converted to oligosaccharides of structural variants using combinatorial enzyme approach. The digestive products were separated by preparative scale chromatographic Amberlite XAD-2 column. Fractions containing feruloyl oligosaccharides (FOS) were isolated, pooled, freeze-dried, and demonstrated to possess antimicrobial activity. The FOS suppressed cell growth of the test organism ATCC 8739 E. coli with a MIC value of 0.028% (w/v, 35˚C, 24 hr). The antimicrobial action was observed exceeding 72 hr of culture incubation. The FOS product could be a useful source of prebiotics or preservatives. The present results further confirm the science and application of the concept of combinatorial enzyme technique.