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Q. R. Chen, L. Zhang, P. W. Luther and A. J. Mixson, “Optimal Transfection with the HK Polymer Depends on Its Degree of Branching and the pH of Endocytic Vesicles,” Nucleic Acids Research, Vol. 30, No. 6, 2002, pp. 1338-1345. doi:10.1093/nar/30.6.1338

has been cited by the following article:

• TITLE: Reducible Poly (2-Dimethylaminoethyl) Methacrylate-Block-Polyvinylimidazole: Synthesis, Transfection Activity in Vitro

  AUTHORS: Bozhang Yu

  KEYWORDS: Reducible Polymer; Gene Expression; Transfection; Cytotoxicity

  JOURNAL NAME: Journal of Biomaterials and Nanobiotechnology, Vol.3 No.1, January 12, 2012

  ABSTRACT: Reducible or imidazolyl polycations of block poly(imidazole/2-dimethyl aminoethyl) are of promising in gene delivery. Dimeric poly(2-dimethyl aminoethyl) methacrylate-block-polyvinylimidazole (rDPDMAEMAIM) and reducible poly (2-dimethylaminoethyl) methacrylate (rDPDMAEMA) with single disulfide bond in the backbone was synthesized by oxidizing their dithioester-terminated polymers. The polyplexes sizes, rDPDMAEMAIM/pDNA and rDPDMAEMA/ pDNA (plasmid DNA) are in the ranges of 100 nm - 150 nm at the weight ratio of 12:1, and the zeta potential of rDPDMAEMAIM/pDNA from 9.6 mV to 22.7 mV in PBS solutions increases with their weight ratios of 1:1 to 18:1. The results show that the rDPDMAEMAIM/pDNA polyplexes have higher transfection activity and lower cytotoxicity than that of rDPDMAEMAIM/pDNA against 293T cells in vitro in the presence of serum, indicating that the PDMAEMAIM present a promising nonviral gene vector.