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K. Miyata, Y. Kakizawa, N. Nishiyama, A. Harada, Y. Yamasaki, H. Koyama and K. Kataoka, “Block Catiomer Polyplexes with Regulated Densities of Charge and Disulfide Cross-Linking Directed to Enhance Gene Expression,” Journal of the American Chemical Society, Vol. 126, No. 8, 2004, pp. 2355-2361. doi:10.1021/ja0379666

has been cited by the following article:

• TITLE: Reducible Poly (2-Dimethylaminoethyl) Methacrylate-Block-Polyvinylimidazole: Synthesis, Transfection Activity in Vitro

  AUTHORS: Bozhang Yu

  KEYWORDS: Reducible Polymer; Gene Expression; Transfection; Cytotoxicity

  JOURNAL NAME: Journal of Biomaterials and Nanobiotechnology, Vol.3 No.1, January 12, 2012

  ABSTRACT: Reducible or imidazolyl polycations of block poly(imidazole/2-dimethyl aminoethyl) are of promising in gene delivery. Dimeric poly(2-dimethyl aminoethyl) methacrylate-block-polyvinylimidazole (rDPDMAEMAIM) and reducible poly (2-dimethylaminoethyl) methacrylate (rDPDMAEMA) with single disulfide bond in the backbone was synthesized by oxidizing their dithioester-terminated polymers. The polyplexes sizes, rDPDMAEMAIM/pDNA and rDPDMAEMA/ pDNA (plasmid DNA) are in the ranges of 100 nm - 150 nm at the weight ratio of 12:1, and the zeta potential of rDPDMAEMAIM/pDNA from 9.6 mV to 22.7 mV in PBS solutions increases with their weight ratios of 1:1 to 18:1. The results show that the rDPDMAEMAIM/pDNA polyplexes have higher transfection activity and lower cytotoxicity than that of rDPDMAEMAIM/pDNA against 293T cells in vitro in the presence of serum, indicating that the PDMAEMAIM present a promising nonviral gene vector.