TITLE:
Identifying microRNA-Target Gene Pairs in Luminal B Breast Cancer Using Integrated Analysis of miRNA and Transcriptome Profiles
AUTHORS:
Kayalvili Ulaganathan, Raviteja Reddy Alipeddi, Surekha Rani Hanumanth
KEYWORDS:
Breast Cancer, Luminal B, miRNA, mRNA, TCGA
JOURNAL NAME:
Advances in Breast Cancer Research,
Vol.13 No.4,
September
23,
2024
ABSTRACT: Dysregulation of post-transcriptional regulation of gene expression has been found to influence various human disorders. Aberrant miRNA-based regulation of gene expression has been found to be associated with different cancers, including breast cancers. Very little information is available on the effect of dysregulation of miRNA-mediated regulation on luminal B breast cancer. This study was aimed at comprehending the regulation of gene expression through miRNA in luminal B breast cancers by comprehensive analysis of miRNA and mRNA expression data together. Negatively regulated miRNA-target gene pairs were identified, and the target genes were functionally enriched to identify critical pathways associated with luminal B breast cancer. Further, the prognostic significance of these miRNAs and target gene pairs was assessed to identify genes with prognostic value in luminal B breast cancer. A total of 266 differentially expressed miRNAs and 164 dysregulated miRNA-target gene pairs were identified. Four genes, including SRP9, DSN1, RACGAP1, and SLC10A6, and one miRNA, hsa-mir-421, showed significant influence on the prognosis of patients with luminal B breast cancer. Through additional experimental examination of these findings, a deeper comprehension of miRNA-based post-transcriptional regulation in luminal B breast tumors will be possible.