TITLE:
Antioxidative and Neuroprotective Activities of the Pre-Germinated Brown Rice Extract
AUTHORS:
Rungtip Soi-ampornkul, Sarawut Junnu, Surin Kanyok, Sompong Liammongkolkul, Wanphen Katanyoo, Supranee Umpornsirirat
KEYWORDS:
Pre-Germinated Brown Rice; SK-N-SH Cells; Antioxidant; GABA; Apoptosis; DNA Fragmentation
JOURNAL NAME:
Food and Nutrition Sciences,
Vol.3 No.1,
January
17,
2012
ABSTRACT: We evaluated the effects of pre-germinated brown rice extract (PGBR ex) with enhanced levels of GABA on proliferation and apoptosis of neuronal SK-N-SH cells line. Firstly, we used HPLC methods to study the level of γ-aminobutyric acid (GABA) in all rice extracts. We found that the concentration of GABA in the PGBR ex were 3 and 8 times higher than the GABA concentration in non-germinated brown rice (BR ex) and white rice (WR ex) compared with the stan- dard GABA respectively. Next we study the protective effects of brown rice extract by investigating various methods, we found that the effects of dose-dependent study by treated with PGBR ex, BR ex and WR ex at (0 - 4000 μg/ml). The data from MTT assay showed that the higher concentration of all rice extracts were not induced toxicity to SK-N-SH cells. To test the protective effect by study the viability of SK-N-SH cells. These results showed that PGBR ex and BR ex can protect cells by significantly increase cells survival up to 29.3% ± 0.01% and13.4% ± 0.07 % (p 56.9% ± 0.02 % (p