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S. Toyokuni, T. Itani, Y. Morimitsu, K. Okada, M. Ozeki, S. Kondo, et al., “Protective Effect of Colored Rice over White Rice on Fenton Reaction-Based Renal Lipid Peroxidation in Rats,” Free Radical Research Communicaitions, Vol. 36, No. 5, 2002, pp. 583-592. doi.10.1080/10715760290025960

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• TITLE: Antioxidative and Neuroprotective Activities of the Pre-Germinated Brown Rice Extract

  AUTHORS: Rungtip Soi-ampornkul, Sarawut Junnu, Surin Kanyok, Sompong Liammongkolkul, Wanphen Katanyoo, Supranee Umpornsirirat

  KEYWORDS: Pre-Germinated Brown Rice; SK-N-SH Cells; Antioxidant; GABA; Apoptosis; DNA Fragmentation

  JOURNAL NAME: Food and Nutrition Sciences, Vol.3 No.1, January 17, 2012

  ABSTRACT: We evaluated the effects of pre-germinated brown rice extract (PGBR ex) with enhanced levels of GABA on proliferation and apoptosis of neuronal SK-N-SH cells line. Firstly, we used HPLC methods to study the level of γ-aminobutyric acid (GABA) in all rice extracts. We found that the concentration of GABA in the PGBR ex were 3 and 8 times higher than the GABA concentration in non-germinated brown rice (BR ex) and white rice (WR ex) compared with the stan- dard GABA respectively. Next we study the protective effects of brown rice extract by investigating various methods, we found that the effects of dose-dependent study by treated with PGBR ex, BR ex and WR ex at (0 - 4000 μg/ml). The data from MTT assay showed that the higher concentration of all rice extracts were not induced toxicity to SK-N-SH cells. To test the protective effect by study the viability of SK-N-SH cells. These results showed that PGBR ex and BR ex can protect cells by significantly increase cells survival up to 29.3% ± 0.01% and13.4% ± 0.07 % (p 56.9% ± 0.02 % (p