TITLE:
Construction of Electrochemical Biosensors Based on the CRISPR/Cas12 System for Applications
AUTHORS:
Hongqu Liang, Liu Huang, Peng Yan, Fa Huang, Hongchao Zhang, Qisheng Luo
KEYWORDS:
CRISPR, Cas12, Electrochemical Biosensor
JOURNAL NAME:
Materials Sciences and Applications,
Vol.15 No.9,
September
3,
2024
ABSTRACT: The current major issue in improving detection sensitivity and selectivity is to design an electrochemical sensor that does not require PCR amplification for nucleic acid identification and measurement. Because of their great sensitivity, precision, and simplicity of downsizing, electrochemical biosensors have emerged as a research hotspot in the field of nucleic acid detection. The CRISPR/Cas12 system has emerged as a potent tool for nucleic acid detection due to its powerful cleavage activity and selectivity. Specific electrode changes combined with the CRISPR/Cas12 system can greatly improve the performance of electrochemical biosensors. In this study, the design concepts of electrochemical biosensors based on the CRISPR/Cas12 system and their application advancements in nucleic acid detection are discussed.