TITLE:
Near-Infrared Spectroscopic Analysis of Hemoglobin with Stability Based on Human Hemolysates Samples
AUTHORS:
Tao Pan, Wenjie Huang, Zhenyao Liu, Lijun Yao
KEYWORDS:
Hemolysates; HGB; NIR Spectroscopy; PLS; Stability
JOURNAL NAME:
American Journal of Analytical Chemistry,
Vol.3 No.1,
January
5,
2012
ABSTRACT: Near-infrared (NIR) spectroscopy combined with the partial least-squares (PLS) regression was successfully applied for the rapid quantitative analysis of hemoglobin (HGB) based on human hemolysates samples. Based on the varied divisions for the calibration and prediction sets, an effective modeling approach using stable model parameters was proposed. Among 255 samples, 80 were randomly selected as the validation set. The remaining 175 samples were divided into the calibration set (110 samples) and the prediction set (65 samples) for a total of 30 times with certain similarities based on partial least squares cross-validation predictive basis (PLSPB). The optimal PLS factor was 8, the modeling effects M-SEPAve, M-RP,Ave, M-SEPStd and M-RP,Std were 3.84g/L, 0.967, 0.16g/L and 0.006, respectively, the validation effects V-SEP, V-RP and V-RSEP were 3.59g/L, 0.980 and 2.7%, respectively. It indicated that the method has high prediction precision and well stability. The results show that NIR spectroscopy of hemolysates is accurate to HGB’s determination, and it is hopeful to be applied to clinic.