TITLE:
Preclinical Verification of Modulated Electro-Hyperthermia —Part I. In Vitro Research
AUTHORS:
Andras Szasz
KEYWORDS:
Modulated Electro-Hyperthermia, mEHT, In Silico Calculations, Phantom Measurement, In Vitro Experiments, Thermal Effects, Nonthermal Processes, Apoptosis
JOURNAL NAME:
International Journal of Clinical Medicine,
Vol.15 No.7,
July
29,
2024
ABSTRACT: Modulated electro-hyperthermia (mEHT) targets tissue’s natural electric and thermal heterogeneities to heat the cancer cells selectively. The applied 13.56 MHz radiofrequency (RF) is a carrier of the low-frequency modulation. The high-frequency part was chosen to select the malignant lesion using the specialties of the tumor: the higher conductivity and dielectric constant of the tumor than its host. The electric field selects the tumor, and the low-frequency amplitude modulation polarizes and excites the transmembrane proteins of the malignant cells. The dominant absorption of the energy by the microscopic clusters of the membrane rafts acts like nanoparticle heating. Exciting the membrane produces various apoptotic signals. The processes were modeled using silico and phantom experiments, which proved the concept. The preclinical verification was made in vitro and in vivo, and in the end, clinical proofs validated the method. Our objective is to follow all the development steps from the laboratory to the clinics in a trilogy of articles. This present is the first part, which deals with in silico, phantom, and in vitro research.