TITLE:
Dissecting Multiple Arabidopsis CC-NBS-LRR Proteins Structure and Localization
AUTHORS:
Jianzhong Huang, Xiuying Guan, Xiaoju Zhong, Peng Jia, Hongbin Zhang, Kai Chen, Zhuojun Li, Guangyu Chen, Chuitang Zeng
KEYWORDS:
Arabidopsis, Calcium Permeation Channel, Pentamer, Plasma Membrane
JOURNAL NAME:
Journal of Biosciences and Medicines,
Vol.12 No.7,
July
15,
2024
ABSTRACT: NBS-LRR (nucleotide binding sites and leucine rich repeat) protein plays a crucial role as sentries and as defense activators in plants. The structure and function of NBS-LRR proteins are closely related. Previous articles have announced that the activated ZAR1 (HopZ-Activated Resistance 1) forms a pentamer in the plasma membrane, which is a calcium permeable channel that can trigger plant immune signaling and cell death. However, the structure of galore NBS-LRRs in Arabidopsis is not yet clear. The functional sites of distinct NBS-LRR in cells may vary. In addition, identifying pathogens and activating defense regions may occur in different subcellular compartments. Therefore, dissecting the specific structure and positioning of NBS-LRRs is an indispensable step in understanding their functions. In this article, we exploit AlphaFold to predict the structure of some designed NBS-LRRs, and utilize Agroinfiltration transient expression system, combined with biochemical fractionation, to dissect the localization of these NBS-LRR receptors from Arabidopsis. Structural data indicates that the identified NBS-LRRs share analogous conformation. Membrane fractionation assay demonstrates these NBS-LRRs are mainly associated with the membrane. These data show that the Ca2+-permeable channel activity may be evolutionarily conserved in NBS-LRR of Arabidopsis, and this study provides some reference clues for analyzing the structure and localization patterns of other plant immune receptors.