TITLE:
Detection of Bisphenol A (BPA) in Plastic Bottles Using Vertical Cultivation at Various Temperatures
AUTHORS:
Karwan Alkaradaghi, Tara Ali, Nadhir Al-Ansari, Polla Khanaqa, Jan Laue
KEYWORDS:
Vertical Cultivation, Allium, Bisphenol A, Plastic Bottles, Scanning Electron Microscope (SEM)
JOURNAL NAME:
Journal of Environmental Protection,
Vol.15 No.6,
June
19,
2024
ABSTRACT: Polycarbonate plastics containing bisphenol A (BPA) used to manufacture drinking water bottles. Kurdistan region in northern Iraq is a developed area with increased pollution from plastic bottles. Trace amounts of BPA have been detected in bottled water samples. The absorption of BPA was measured with HPLC using a vertical cultivation system with Bulbs of the Allium Cepa plant planted in these plastic bottles with monitored growth. Vertical cultivation was found to have a low level of BPA in the plant cells, making it a safe cultivation method under specific climate conditions. The mean concentration of BPA in vertical cultivation is 0.19 ug/ml (3.8 ng for a 20 uL injection), and the Limit of Quantification (LOQ) is 0.63 ug/ml (12.7 ng for 20 uL injection). While Scanning Electron Microscope (SEM) shows that the concentrations are relatively low in water samples stored at room temperature compared to those exposed to direct sunlight (40°C) and water bottle samples stored at (-4°C), The correlation coefficients were found to be good (0.9992). SEM is used for plastic bottle samples stored at different temperatures. The images identify compound decay and explore the morphology of BPA in manufactured plastic materials.