TITLE:
Validation of a Method for Characterization of Ethanol in Water by HS-GC-FID to Serve the Traceability of Halal Measurements
AUTHORS:
Adel B. Shehata, Abdulrahman R. Al Askar, Mohammed A. Al Rasheed, Abdulrahman M. Al Zahrany, Fahd A. Al Kharraa, Sowailem A. Al Sowailem
KEYWORDS:
Ethanol, HS-GC-FID, Validation, Linearity, Precision, Bias
JOURNAL NAME:
Green and Sustainable Chemistry,
Vol.14 No.2,
May
21,
2024
ABSTRACT: The
determination of the ethanol content in food products is of fundamental
importance for HALAL certification. In this work, an analytical method for the
determination of ethanol in water by headspace gas chromatography with flame
ionization detector (HS-GC-FID) has been developed and validated for the use in characterization of ethanol reference
materials. The validation study was carried out in
the linear calibration range 100 - 1500
mg/kg using the NIST SRM 2900, nominal 95.6%. The studied performance
characteristics of the method were the limit of detection, LOD, the limit of
quantification LOQ, selectivity, linearity,
precision, recovery and bias. The validation results showed that the
method is selective, precise, accurate and free from any significant bias. The LOD and LOQ were 1.27 and 3.86 mg/kg respectively and the estimated expanded uncertainty was 2% indicating that the method is fit for the
purpose of certification of ethanol in water reference materials.