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Minana, R., Sancho-Tello, M., Climent, E., Segui, J.M., Renau-Piqueras, J. and Guerri, C. (1998) Intracellular location, temporal expression, and polysialylation of neural cell adhesion molecule in astrocytes in primary culture. Glia, 24, 415-427. doi:10.1002/(SICI)1098-1136(199812)24:4<415::AID-GLIA7>3.0.CO;2-A

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• TITLE: Bone marrow increases human islets insulin positive cells in co-culture: quantification with flow cytometry

  AUTHORS: Zhengke Wang, Fang Xiong, Mary Hassani, John Z. Q. Luo, LuGuang Luo

  KEYWORDS: Bone marrow; Human Islets; Flow Cytometry

  JOURNAL NAME: Journal of Diabetes Mellitus, Vol.1 No.4, November 8, 2011

  ABSTRACT: We have previously demonstrated that allogeneic human bone marrow (BM) supports human islet function and longevity in vitro. We hypothesize that BM supporting human islets may include to increase β-cell in cultured islets. In this study, we developed a method to quantify insulin-producing β cells from cultured islets by using immunofluorescent staining and flow cytometry analysis to explore this possibility. The results show that human islets cocultured with BM for 39 days contained a significantly higher number of insulin-positive β cells (42.3% ± 4.5%) compared to the islet-only cultures (1.15% ± 0.78%), and increased insulin release levels evaluated by ELISA is consistent with increased β cells in same culture condition. Human islet culture with BM significantly increase β-cells while islet only culture lost β-cells in same culture period supports the possibility of BM increasing β-cells in cultured islets.