TITLE:
Decoding Retinoblastoma: Differential Gene Expression
AUTHORS:
Ahmed Jasim Mahmood Al-Mashhadani, Franko Shehaj, Lianhong Zhou
KEYWORDS:
Retinoblastoma Gene Expression, In Silico Study, Differentially Expressed Genes, Chemoresistance, Immune Response, Computational Biology
JOURNAL NAME:
International Journal of Clinical Medicine,
Vol.15 No.4,
April
22,
2024
ABSTRACT: Background: Retinoblastoma, the most common intraocular pediatric cancer, presents complexities in its genetic landscape that necessitate a deeper understanding for improved therapeutic interventions. This study leverages computational tools to dissect the differential gene expression profiles in retinoblastoma. Methods: Employing an in silico approach, we analyzed gene expression data from public repositories by applying rigorous statistical models, including limma and de seq 2, for identifying differentially expressed genes DEGs. Our findings were validated through cross-referencing with independent datasets and existing literature. We further employed functional annotation and pathway analysis to elucidate the biological significance of these DEGs. Results: Our computational analysis confirmed the dysregulation of key retinoblastoma-associated genes. In comparison to normal retinal tissue, RB1 exhibited a 2.5-fold increase in expression (adjusted p Conclusions: Our analysis reinforces the critical genetic alterations known in retinoblastoma and unveils new avenues for research into the disease’s molecular basis. The discovery of chemoresistance markers and immune-related genes opens potential pathways for personalized treatment strategies. The study’s outcomes emphasize the power of in silico analyses in unraveling complex cancer genomics.