TITLE:
3,6-Bis-β-Dicarbonylsubstituted Carbazoles Bearing N-Spacers and Their Eu(III) Complexes as Immunofluorescent Labelling Agents
AUTHORS:
Dmitry E. Pugachev, Georgy V. Zatonsky, Tatyana S. Kostryukova, Anna G. Shubina, Nikolay V. Vasiliev
KEYWORDS:
Fluorescence Immunoassay, Fluorinated β-Diketones, Carbazole, Europium Complexes, Streptavidin, Nanodispersions
JOURNAL NAME:
International Journal of Organic Chemistry,
Vol.14 No.1,
March
15,
2024
ABSTRACT: New reagents for immunofluorescence analysis of carbazole series containing fluorinated β-dicarbonyl fragments and carboxylic substituent groups separated by spacers of different lengths from the light-gathering carbazole scaffold have been developed. The markers in complex with Eu3+ ions possess stability in the aqueous phase, intense and prolonged luminescence (τ 550 - 570 μs) with characteristic emission maxima in the region of 615 nm and excitation wavelengths in the region of 380 - 390 nm, which distinguishes them from most of the analogs used. In the study of marker conjugation with streptavidin, a reagent containing 4 - 5 europium labeling complexes based on spacer-containing carbazole tetraketone was obtained. The marker-doped silicate nanoparticles exhibit intense and long-lived luminescence in the characteristic region.