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Spector, D., Legendre, A.M., Wheat, J., Bemis, D., Rohrbach, B., Taboada, J. and Durkin, M. (2008) Antigen and antibody testing for the diagnosis of blastomycosis in dogs. Journal of Veterinary Internal Medicine, 22, 839-843. doi:10.1111/j.1939-1676.2008.0107.x

has been cited by the following article:

• TITLE: Comparison of antibody detection with Blastomycesdermatitidis yeast lysate antigens in serum specimens from immunized rabbits and infected dogs

  AUTHORS: Will Christenson, Rachel Horton, Kayla Campbell, Kelly Meacham, Amber Schroeder, Gene M. Scalarone

  KEYWORDS: ELISA; Blastomycesdermatitidis; Antibody Detection; Lysate Antigens

  JOURNAL NAME: Open Journal of Immunology, Vol.1 No.3, December 31, 2011

  ABSTRACT: This present study was designed to evaluate B. dermatitidis antigens, prepared from two isolates (B5896, 597), when the yeast cells were allowed to lyse in distilled water for one day or seven days. The indirect enzyme-linked immunosorbent assay (ELISA) was used to determine the ability of the lysate reagents to detect antibodies in 30 rabbit and 30 dog serum specimens. Mean absorbance values with B5896 lysate antigen ranged from 1.637 (day 1) to 1.461 (day 7) and absorbance values with 597 antigen ranged from 1.579(day 1) to 1.396 (day 7) with the serum specimens from immunized rabbits. Serum specimens from infected dogs yielded absorbance values ranging from 1.672 (day 1) to 1.763 (day 7) with the B5896 and values ranging from 1.909 (day 1) to 1.224 (day 7) with the 597. Optimal reactivity was obtained with the day 1 lysate using both lysate antigens against the rabbit sera and with the 597 antigen against the dog sera. Slightly greater reactivity was evidenced with the day 7 B5896 antigen when the dog sera was tested. Comparative studies are continuing in order to produce an optimal anti-genic preparation for antibody detection in blastomycosis.