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Gomez, X., Sanon, S., Zambrano, K., Asquel, S., Bassantes, M., Morales, J.E., Otanez, G., Pomaquero, C., Villaroel, S., Zurita, A., Calvache, C., Celi, K., Contreras, T., Corrales, D., Naciph, M.B., Pena, J. and Caicedo, A. (2021) Key Points for the Development of Antioxidant Cocktails to Prevent Cellular Stress and Damage Caused by Reactive Oxygen Species (ROS) During Manned Space Missions. NPJ microgravity, 35, 1-19.

has been cited by the following article:

• TITLE: Antioxidant Activity and Phytochemical Screening in Acacia rigidula Benth. Leaves

  AUTHORS: Aneth Ochoa-Negrete, Rene Rangel, Marcus Antonius Ynalvez, Ruby A. Ynalvez

  KEYWORDS: Acacia, Plant Extract, Antioxidant Activity, Total Phenolic Acid, HPLC, Gallic Acid

  JOURNAL NAME: Advances in Bioscience and Biotechnology, Vol.15 No.1, January 26, 2024

  ABSTRACT: This study aimed to compare the total phenolic content (TPC) and antioxidant activities of A. rigidula extracts. It also aimed to identify phenolic acids present in the extracts. The 2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), and ferric thiocyanate lipid peroxidation antioxidant assays were performed. High performance liquid chromatography was used to identify phenolic acids. There was no solvent effect on TPC nor on scavenging activities, and inhibition of lipid peroxidation (p > 0.05) among solvent extracts. On the other hand, 1:1:3 water: acetone: methanol extract (10.22 mg GAE/g sample) had significantly higher reducing potential than 50% ethanol extract (EE) (9.259 mg GAE/g sample) (p 0.05). Phenolic fraction designated as fraction 4 had the highest antioxidant activity (p reducing potential, 22.26 mg of GAE/g sample. DPPH scavenging activities of fractions 4 (55.59%) and 5 (55.64%) were significantly higher than the other fractions (p A. rigidula extracts contain gallic, caffeic, vanillic, p-coumaric, salicylic acids and vanillin.