TITLE:
Assessing Genetic Structure and Relatedness of Jerusalem Artichoke (Helianthus tuberosus L.) Germplasm with RAPD, ISSR and SRAP Markers
AUTHORS:
Preeya Puangsomlee Wangsomnuk, Sudarat Khampa, Sanun Jogloy, Trin Srivong, Aran Patanothai, Yong-Bi Fu
KEYWORDS:
Jerusalem Artichoke, genetic Structure, Germplasm Management, RAPD, ISSR, SRAP
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.2 No.6,
December
5,
2011
ABSTRACT: Jerusalem artichoke (Helianthus tuberosus L.) is an old tuber crop with a recently renewed interest in multipurpose improvement, but little effort has been made to characterize its genetic resources. A study was conducted to assess genetic structure and genetic relatedness of 47 diverse Jerusalem artichoke accessions using RAPD, ISSR and SRAP markers. A total of 296 (87.1%) polymorphic bands were detected from 13 RAPD markers; 92 (80%) from six ISSR primers; and 194 (88.6%) for nine combinations of SRAP primers. Five optimal clusters were inferred by the STRUCTURE program from the RAPD or ISSR data, while six optimal clusters were found from the SRAP data or combined marker data. Significant linear relationships between the distance matrices for all pairs of individual accessions were detected for all marker pairs and the estimated correlation coefficient was 0.40 for RAPD-ISSR, 0.53 for RAPD-SRAP, and 0.43 for ISSR-SRAP. Based on the combined data, the neighbor-joining clustering of the 47 accessions matched closely with those inferred from the STRUCTURE program. Three ancestral groups were observed for the Canadian germplasm. Most diverse germplasm harbored in the USA collection. These findings not only reveal the compatible patterns of genetic structure and relatedness inferred with three marker types, but also are useful for managing Jerusalem artichoke germplasm and utilizing diverse germplasm for genetic improvement.