TITLE:
In Vitro Efficacy Assessment of Fungicides against Botrytis gladiolorum Causing Gladiolus Leaf Blight
AUTHORS:
M. A. Rahaman, M. S. M. Chowdhury, M. R. Islam, N. Sultana, M. R. Ali, N. Akhter, F. M. Aminuzzaman
KEYWORDS:
Leaf Blight, Gladiolus, Botrytis gladilorum, Fungicides, Management
JOURNAL NAME:
Open Access Library Journal,
Vol.10 No.12,
December
28,
2023
ABSTRACT: Leaf blight of gladiolus caused by Botrytis gladiolorum is widely distributed as a major disease in gladiolus growing districts of Bangladesh where farmers usually used fungicides to manage diseases. Nowadays, it is very important to find out the more effective fungicides against Botrytis gladiolorum (Bg). An experiment was conducted at the Mycology laboratory, Department of Plant Pathology, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh to find out the efficacy of selected ten fungicides against Botrytis gladiolorum (Bg) causing gladiolus leaf blight. The aim of the study was to find out the more effective fungicides against Botrytis gladiolorum (Bg) in laboratory. Infected leaves samples were collected from gladiolus farmers’ field and brought to the laboratory for study. Ten selected fungicides were used against the colony growth of Bg. The fungicides were Tilt 250 EC, Score 250 EC, Folicure 250 EC, Amistar top 325 SC, Nativo 75 WG, Trooper 75 WP, Autostin 50 WDG, Differ 300 EC, Indofil M-45 80 WP and Contaf 5 EC. The fungicides were applied at the rate of 100 ppm, 200 ppm and 300 ppm. Among ten fungicides, Contaf 5 EC gave the best results in arresting radial mycelia growth and it was nil (00.00 mm) after 5 DAI in 100 ppm which was statistically similar with Indofil 80 WP treated (00.00 mm) plate and the inhibition of growth was 100%. At 15 DAI the growth inhibition (87.34%) was highest in Contaf 5 EC treated plate followed by Score 250 EC (78.02%) and Autostin 50 WDG (72.34%) respectively. In 200 ppm Contaf 5 EC showed the best performance against mycelial growth at 15 DAI and gave 100% growth inhibition which was statistically similar to Score 250 EC (100% inhibition) followed by Autostin 50 WDG (83.04) and Folicure 250 EC (82.90%). In 300 ppm Contaf 5 EC showed the best performance at 15 DAI against mycelial growth and showed 100% inhibition which was statistically similar to Score 250 EC (100% inhibition), Autostin 50 WDG, Tilt 250 EC and Folicure 250 EC.