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Lorimer, S.D., Perry, N.B., Foster, L.M., Burgess, E.J., Douch, P.G.C., Hamilton, M.C., Donaghy, M.J. and McGregor, R.A. (1996) A Nematode Larval Motility Inhibition Assay for Screening Plant Extracts and Natural Products. Journal of Agricultural and Food Chemistry, 44, 2842-2845. doi:10.1021/jf9602176

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• TITLE: Decision-making by nematodes in complex microfluidic mazes

  AUTHORS: Santosh Pandey, Andrew Joseph, Roy Lycke, Archana Parashar

  KEYWORDS: C. elegans; Navigation; Maze; Microorganism; Microfluidics

  JOURNAL NAME: Advances in Bioscience and Biotechnology, Vol.2 No.6, December 2, 2011

  ABSTRACT: Nematodes are microscopic, soil-dwelling worms that navigate through soil particles in search of food or a suitable host. Most nematode species employ a myriad of physical and chemical cues that define their navigation strategies. Here, we demonstrate a microfluidic method to observe and characterize the physical aspects of nematode navigation at real-time. The microfluidic devices comprise a series of interconnected T-maze or cylindrical structures of varying geometry. At each physical intersection, nematodes are given the choice to migrate left or right. We found that this decision-making of nematodes is influenced by the angle of intersection of T-maze structures. We further showed that nematodes can be passively directed to move in a linear direction by carefully adjusting the position and spacing of cylindrical obstacles in its path. The experiments were conducted on two nematodes (non-parasitic C. elegans and pigparasitic Oesophagostomum dentatum) and in the absence of any chemical or electrical stimulants.