TITLE:
Antibacterial Properties of Crateva adansonii (Capparidaceae) on Strains Isolated from Chronic Wounds Diagnosed in the Commune of Ouinhi in 2021
AUTHORS:
Zinsou Franck Maurille Mignanwandé, Hervé Kouessivi Janvier Bokossa, Jules Afle, Dagbédji Damien Toffa, Carine Zinsou, Wilfrid Hinnoutondji Kpètèhoto, Rafiatou Ba, Esai Gimatal Anagonou, Roch Christian Johnson
KEYWORDS:
Crateva adansonii, Antimicrobial Activities, Chronic Wound Strains, Benin
JOURNAL NAME:
Pharmacology & Pharmacy,
Vol.14 No.11,
November
28,
2023
ABSTRACT: Plants have always been used by people for therapeutic purposes. They are
still the main source of therapeutic substances in developing countries. Crateva adansonii, a member of the
Capparidaceae family, is a medicinal plant with antibacterial properties used
in Benin. The aim of this study was to assess the efficacy of an aqueous
extract of C. adansonii on bacterial strains isolated from chronic
wounds in the Ouinhi population. To achieve this, the bacterial flora
present in chronic wounds was identified using the Ikram method (2014) coupled with the API Remoel One method. The
antibacterial properties of the aqueous extract of C. adansonii on the microbial
strains isolated were then assessed by determining the Inhibition Diameters
(ID), the Minimum Inhibitory Concentrations (MIC) and finally the Minimum
Bactericidal Concentrations (MBC). A total of eighty (80) strains were isolated
and identified on the basis of morphological, cultural and biochemical
characteristics. The species S. Aureus species accounted for
the largest proportion (67.5%). Other species such as Listeria sp, Pseudomonas proteus, S. epidermidis and Bacillus cereus, Citrobacter freundii, Steno maltophila; Axin calcoaceticus, E. coli, K. pneumonia, Lem. richardii, Salmonella paratyphi A, Salmonella sp, Shigella spwere determined in variable proportions. At a concentration of 10 mg/ml, only
S. aureus was sensitive to contact with the extract. However, at 20 mg/ml, 89%
of strains were sensitive and 11% very sensitive. The highly sensitive strains are Salmonella sp and E. coli. The Minimum Inhibitory
Concentration (MIC) and Minimum Bactericidal Concentration (MBC) are 20
mg/ml and 40 mg/ml respectively. The MBC/MIC ratio of the aqueous mixture of Crateva adansonii (Capparidaceae)
varied from 1.2 to 2, with a bactericidal effect on 100% of the strains tested.