TITLE:
Morphology and Molecular Identification of Dry Fish Fungus Cunninghamella blakesleeana from Small Indigenous Fish “Kachki” Corica soborna (Hamilton 1822) in Bangladesh
AUTHORS:
Abdullah Al Masud, Ismot Ara, Nuhu Alam
KEYWORDS:
Culture Media, Molecular Identification, Temperature, pH, Vegetative Growth
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.14 No.11,
November
24,
2023
ABSTRACT: The present experiment was conducted to investigate
a dry fish fungus, Cunnighamella
blakesleeana, which was identified from the infected part of the Corica
soborna, locally named as Kachki fish. Mycelium
was hyaline, often with granular
content, and conidiophores were erected, with verticillate or solitary branches. Zygospores were globose,
tuberculate, suspensors equal, smooth,
hyaline and heterothallic. Using ITS4 and ITS5 primers, the 740 bp-long
ITS region was amplified and sequenced. The ITS region sequences had reciprocal
homologies of 98% to 100%. The findings showed that several species of C.
blakesleeana fall into the same cluster. It has been determined by molecular data that the fungus we had studied was C.
blakesleeana. The maximum mycelial
growth (95.33 mm) was observed in the PDA medium, followed by the PSA
medium, and the lowest growth (65.50 mm) was measured in the HPA medium in the
study of the impact of culture media on the mycelial growth of C. blakesleeana. The influence of temperature on the
radial mycelial growth of C. blakesleeana on PDA medium was
investigated through five different temperatures. Although
pH is a crucial factor in understanding the ecology of spoilage fungus, the
highest mycelial growth of C. blakesleeana (88.25 mm) was seen at pH 7,
followed by pH 8 and pH 6, while pH 9 was revealed
to have the lowest mycelial growth. The outcome suggested that C. blakesleeana thrived in neutral environments.