TITLE:
A validated, related substance, GC method for 1,4-cyclohexanedione mono-ethylene Ketal
AUTHORS:
B. Karthikeyan, V. Srinivasan
KEYWORDS:
Column Gas Chromatography; 1, 4-Cyclohexanedione Mono-Ethylene Ketal; 1, 4-Cyclohexanedione Bis (Ethylene Ketal); Validation
JOURNAL NAME:
Journal of Biophysical Chemistry,
Vol.1 No.1,
June
1,
2010
ABSTRACT: ABSTRACT
A simple, economic, and time-efficient related substance, GC method has been developed for the analysis of 1,4-Cyclohexanedione mono-
ethylene ketal(will be specified as ketal) in the presence of a potential impurity 1,4-cyclohexa-
nedione bis (ethylene ketal) [will be specified as diketal]. Successful chromatographic separa- tion of the ketal from the impurity was achieved on a DBWAX ETR, 30 m x 0.32 mm x 1.0μ FT column with nitrogen as carrier gas and FID detector. The method was validated for linearity, accuracy, precision, and specificity and can be used for quality control during manufacture of ketal. A validated GC method is reported for the ketal for the first time.