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Ebensen, T., Paukner, S., Link, C., Kudela, P., de Domenico, C., Lubitz, W. and Guzman, C. A. (2004) Bac-terial ghosts are an efficient delivery system for DNA vac-cines. Journal of Immunology, 172, 6858-6865.

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• TITLE: A Plasmid vector encoding functional human keratinocyte growth factor gene in vitro—Functional human KGF gene expression in vitro

  AUTHORS: Lin Qiu, Chunbao Guo

  KEYWORDS: Human Embryo Lung Fibroblast; Gene Clone; Reverse Transcriptage Polymerase Chain Reaction; Eukaryotic Expression Vector

  JOURNAL NAME: Journal of Biophysical Chemistry, Vol.1 No.1, June 1, 2010

  ABSTRACT: In this study, we cloned human KGF (hKGF) genes using RT-PCR techniques and developed a eukaryotic expression plasmid vector capable of directing the expression of functional hKGF. Monolayer culture of human embryo lung fibro-blast (HLF) was used for isolation of total RNA. Then the total RNA was purified and reverse- transcribed into cDNA using an oligo (dT) primer. A full PCR fragment for hKGF was generated and cloned. Restriction digestion and nucleo-tide sequence analysis validated the complete hKGF transcription. The hKGF cDNA fragment was inserted into pEGFP-C2 vector by means of recombinant DNA technology and verified by restriction analysis and sequencing. We have constructed pEGFP-C2-hKGF encoding the green fluorescent protein (GFP). Furthermore, hKGF had the effect on AEC II proliferation. These results suggest that the potential appli-cation of a hKGF plasmid of gene expression should be useful for sustained AEC proliferation, and its in vivo efficacy needs to be validated. Keywords: