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Yi, E. S., Williams, S. T., Lee, H., Malicki, D. M., Chin, E. M, Yin, S., Tarpley, J. and Ulich, T. R. (1996) Kerati-nocyte growth factor ameliorates radiation- and bleomycin- induced lung injury and mortality. American Journal of Pathology, 149, 1963-1970.

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• TITLE: A Plasmid vector encoding functional human keratinocyte growth factor gene in vitro—Functional human KGF gene expression in vitro

  AUTHORS: Lin Qiu, Chunbao Guo

  KEYWORDS: Human Embryo Lung Fibroblast; Gene Clone; Reverse Transcriptage Polymerase Chain Reaction; Eukaryotic Expression Vector

  JOURNAL NAME: Journal of Biophysical Chemistry, Vol.1 No.1, June 1, 2010

  ABSTRACT: In this study, we cloned human KGF (hKGF) genes using RT-PCR techniques and developed a eukaryotic expression plasmid vector capable of directing the expression of functional hKGF. Monolayer culture of human embryo lung fibro-blast (HLF) was used for isolation of total RNA. Then the total RNA was purified and reverse- transcribed into cDNA using an oligo (dT) primer. A full PCR fragment for hKGF was generated and cloned. Restriction digestion and nucleo-tide sequence analysis validated the complete hKGF transcription. The hKGF cDNA fragment was inserted into pEGFP-C2 vector by means of recombinant DNA technology and verified by restriction analysis and sequencing. We have constructed pEGFP-C2-hKGF encoding the green fluorescent protein (GFP). Furthermore, hKGF had the effect on AEC II proliferation. These results suggest that the potential appli-cation of a hKGF plasmid of gene expression should be useful for sustained AEC proliferation, and its in vivo efficacy needs to be validated. Keywords: