TITLE:
In Vitro Efficacy Assessment of Botanical Extracts against Botrytis gladiolorum Causing Gladiolus Leaf Blight
AUTHORS:
M. A. Rahaman, M. S. M. Chowdhury, M. R. Islam, N. Sultana, M. R. Ali, N. Akhter, F. M. Aminuzzaman
KEYWORDS:
Gladiolus, Leaf Blight, Botrytis gladiolorum, Botanical Extracts, Ecofriendly Management
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.14 No.10,
October
26,
2023
ABSTRACT: An experiment was conducted at the Mycology laboratory, Department of
Plant Pathology, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh to
find out the efficacy of selected botanical extracts against Botrytis
gladiolorum (Bg) causing gladiolus leaf blight. Infected
leaves samples were collected from gladiolus farmers’ field and brought to the
laboratory for study. Ten selected botanicals were used against the colony
growth of (Bg). The botanicals were Mehendi,
Chrysanthemum, Basil (Tulsi), Onion, Neem, Bael, Arjuna, Garlic, Aloevera
(Ghritkumary) and Turmeric. Botanical extracts were applied at the rate of 5%,
10% and 20%. The radial mycelia growth was found minimum (11.60 mm) in garlic
extract treated plate at the dose of 5% at 5 DAI, which was statistically
similar with turmeric extract treated (14.00 mm) plate and the inhibition of
growth was 60% and 51.72%, respectively. Similar trend was found at 10 DAI and
15 DAI. At 15 DAI, garlic and turmeric extract gave the best result against Bg, which was statistically similar with
onion (50.07% inhibition) and mehendi (49.93%). All botanicals showed
significantly different results over control and found effective in reducing
the mycelial growth at the dose of 10%. At 5 DAI, no radial mycelia growth was
found in garlic treated plate, which was statistically similar with onion
treated plate, means that the inhibition of growth was 100%. Similar trend was
also found at 10 DAI, and 15 DAI but at 15 DAI, onion (30.20 mm) gave the
statistically similar results with Garlic (30.10 mm) and the mycelia growth
inhibition was (57.70%) and (57.84%), respectively. In case of 20% dose, garlic
extract showed the best result at 5 DAI and the radial mycelia growth was found
minimum (00.00 mm) which was statistically similar with onion extract treated
(00.00 mm) plate and turmeric extract treated plate. The inhibition of growth
was 100%. At 15 DAI, the
inhibition of fungal growth was found (73.74%), (71.23%) and (66.90%),
respectively with treated by turmeric (18.80 mm), garlic (20.60 mm) and onion
(23.70 mm).